aseptic condition

Under aseptic condition, the synovial membrane of knee joint from New Zealand white rabbits of 6 months of age was incised with a sharp knife, and the synovial cells were isolated by means of tissue explant cultivation and enzyme digestion.

目的探讨兔关节滑膜细胞的体外分离、培养和纯化方法。方法无菌条件下，从6月龄新西兰白兔的膝关节囊内剪取滑膜组织，采用组织块培养法和酶消化法分离滑膜细胞。

METHODS: Femoral bone marrow of the rats was extracted under aseptic condition. Bone marrow mesenchymal stem cells ( MSCs) were isolated with density gradient centrifugation.

方法：无菌抽取大鼠股骨骨髓，采用密度梯度离心法分离骨髓间充质干细胞。

Taking the tender bud under the aseptic condition as the explant, experiments were carried out to study the effects of different exogenous hormones, cane sugar and ascorbic acid on proliferation of cluster buds.

以木本切花植物极美泰洛泊在无菌条件下发芽所得的幼芽为外植体，采用正交设计试验，研究了不同水平的6-BA、NAA外源激素，蔗糖、抗坏血酸对极美泰洛泊幼芽丛芽的增殖效果。

The best drawing materials time is after pollination of green legume 110 days. While legume is cultivated in aseptic condition.

果荚无菌播种培养，以授粉后生长110d的绿果荚为最佳取材时期；

Objective: To explore the preparative method for blood platelet concentrate under the aseptic condition.

目的：探索动脉血小板浓缩液，在无菌情况下的制备方法。

Total RNA was extracted from the pregnancy decidual tissues gained under the aseptic condition and remained a part of fresh organization fixed by 4 ﹪ of paraformaldehyde.

在无菌条件下获取她们的妊娠蜕膜组织，提取总RNA并留取部分新鲜组织用4%的多聚甲醛固定。

Rational design and qualified construction is the basis to realize aseptic condition in clean operating room, while scientific operation management and standardized daily monitoring at the operation state is the guarantee to control contamination in the whole process.

合理的设计，优质的施工是洁净手术部实现无菌环境的前提。科学的运行管理，规范的日常监测的运行状态则是实现全方位、全过程控制污染的保证。

Taken from fetus of non-plan-birth in aseptic condition, limb and costal cartilages were used in 38 clinical cases after being frozen at-80 ℃ and preserved in-196 ℃ liquid nitrogen with 15% dimethylsulfoxside ( DMSO) as cryoprotective agent.

在无菌条件下切取计划外生育引产胎儿的四肢及胸肋部软骨，应用于临床38例。将切取的软骨经15%的二甲基亚砜（DMSO）为低温保护剂。

Isolation and amplification culture of bone marrow stromal cells Acquired bone marrow under aseptic condition; Density gradientcentrifugation;

方法1.无菌穿刺抽取骨髓，密度梯度离心，粘附法提取骨髓基质细胞。

The sufferers use antibiotic eyedrops to eliminate conjunctival sac bacterial before operation, this will keep conjunctival sac in aseptic condition, aseptic operation should be strictly carried out during the operation, antibiotics will be used after operation to prevent occurrence of endophthalmitis.

术前患者应用抗生素滴眼液以消灭结膜囊细菌，使结膜囊处于相对无菌状态，同时术中严格执行无菌操作，术后常规应用抗生素，以预防眼内炎的发生。

Objective : To explore the preparative method for blood platelet concentrate under the aseptic condition.

目的: 探索动脉血小板浓缩液,在无菌情况下的制备方法.

The effects of different concentrations of arsenic on spore germination and rhizoid and prothallium growth of Pteris vittata under aseptic condition were studied.

研究无菌条件下不同浓度砷对蜈蚣草的孢子萌发、假根和原叶体生长发育的影响。

The toxicity of 58 bacterial strains were tested with the bacteria cultural filtered original liquid and concentrated liquid and aseptic black pine seedlings cultured for 60d under the aseptic condition.

以培养60天的无菌黑松切根苗为实验材料，用细菌菌株的培养过滤原液和浓缩液，在无菌条件下，测定了58个菌株培养物的致萎活性。